up:: Career Development & Business MOC
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Info
This MOC focuses on Biological Science MOC and closely associated topics such as Molecular Biology, Neurobiology, Neuroscience, Genetics, and Cellular Biology.
Major tags include:
science
”/molbio
”/neuro
”/genetics
”/cellbio
”/evol
”/anthro
Cell Bio
- A Beginner’s Guide to Cell Culture
- while antibiotics are convenient, strict aeseptic techniques produce consistently reliable cell culture data
- three types of cell lines - finite, continuous or immortalized, and stem cell lines
- the potential of bio hacking in self-directed apoptosis
- software FloJo popular for analyzing flow cytometry data
- single-cell sequencing and its use in drug development
- proliferation assays and common issues with pbmcs
- pbmcs are a source of diagnostic and prognostic biomarkers
- how to decide if we should use magnetic cell separation of FACS to isolate cells
- cytotoxicity assays and common issues with pbmcs
- cytokine production assays and their common issues
- cell isolation, cell separation, and cell sorting can be used for different purposes but are very related
- cancer at a cellular level
- Small Lab Process hacks
- ELISAs
- Chemometec training
Mol Bio
- How to Adapt Centrifuge Time for Protocols
- Using Snapgene for plasmids and cloning
- why use a fluorometer instead of a spectrophotometer
- the VGEF protein and its molecular pathway
- the HER2 protein and pathway
- the 5 properties of genetic codes
- tRNAs and ribosomes
- probes and plotting techniques in molecular biology
- membrane tyrosine kinase
- ion channels
- Troubleshooting Checkpoints for Cloning
- Restriction Cloning
- Identifying quality of DNA prep on structureed
- ICM - Assistante Ingénieur
- ELISAs
- Designing PCR Primers
- DNA replication is a semiconservative process
- Chargaff’s Rule
- Acridine orange
- BCMB 412 Test 3 Study Guide
- BCMB 412 Test 2 Study Guide
- BCMB 412 TEST 1 Study Guide
- BCMB 412 Final Exam Study Guide
Primers
Primers, aka oligonucleotides (oligos), are key in DNA synthesis. They’re small pieces of single-stranded nucleotides, around 5-22 bps in length. Primers must be complementary to DNA strands, which “prime” the strands to allow DNA polymerase to bind and start DNA synthesis
There are DNA and RNA primers, and DNA primers are used in experiments because they’re mot stable. RNA primers are more common in vivo, but DNA primers are used in PCR amplification, DNA sequencing, cloning, etc.
DNA primers vs RNA primers
- Reaction:
- amplification is temp. dependent, requires less proteins
- **replication is enzyme dependent catalytic reaction, requires lots of proteins
- Length:
- 18-24 bps
- **10-20 bps
- Creation:
- chemically syntheized (lab made)
- **primase (type of RNA polymerase) creates them
- Viability:
- longer shelf-life, more stable
- **shorter life, more reactive
- Designing PCR Primers
Neurobiology
Experimental Methods
- ELISAs
- pbmcs are a source of diagnostic and prognostic biomarkers
- Meso Scale Discovery: similar to ELISA, but higher sensitivity, larger detection range, and still efficient at low volume samples. Also has minimized matrix effect, which helps with simultaneous multiplex detection with high precision.