Biological Science MOC

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Info

This MOC focuses on Biological Science MOC and closely associated topics such as Molecular Biology, Neurobiology, Neuroscience, Genetics, and Cellular Biology.
Major tags include:
science
”/molbio
”/neuro
”/genetics
”/cellbio
”/evol
”/anthro

Cell Bio

• A Beginner’s Guide to Cell Culture

• while antibiotics are convenient, strict aeseptic techniques produce consistently reliable cell culture data

• three types of cell lines - finite, continuous or immortalized, and stem cell lines

• the potential of bio hacking in self-directed apoptosis

• software FloJo popular for analyzing flow cytometry data

• single-cell sequencing and its use in drug development

• Small Lab Process hacks

• proliferation assays and common issues with pbmcs

• Prolactin signaling

• pbmcs are a source of diagnostic and prognostic biomarkers

• how to decide if we should use magnetic cell separation of FACS to isolate cells

• gating, forward-scatter cells, side-scatter cells

• ELISAs

• cytotoxicity assays and common issues with pbmcs

• cytokine production assays and their common issues

• class I cytokine receptor

• Chemometec training

• cell line quality control testing for new cells

• cell isolation, cell separation, and cell sorting can be used for different purposes but are very related

• cancer at a cellular level

• 3D cell culture and techniques

Mol Bio

• How to Adapt Centrifuge Time for Protocols
• Using Snapgene for plasmids and cloning
• why use a fluorometer instead of a spectrophotometer
• western blot
• tRNAs and ribosomes
• Troubleshooting Checkpoints for Cloning
• the VGEF protein and its molecular pathway
• the HER2 protein and pathway
• the 5 properties of genetic codes
• southwestern blot
• ribozymes (RNase) enable tertiary structure change via intramolecular interactions
• Restriction Cloning
• probes
• Polymerase chain reaction (PCR)
• membrane tyrosine kinase
• ion channels
• Identifying quality of DNA prep on structureed
• ICM - Assistante Ingénieur
• how to create dna biochips
• ELISAs
• DNA replication is a semiconservative process
• DNA hybridization
• DNA Microarrays
• Designing PCR Primers
• Chargaff’s Rule
• blotting techniques
• BCMB 412 Advanced Molecular Biology and Genomics
• BCMB 412 Test 3 Study Guide
• BCMB 412 Test 2 Study Guide
• BCMB 412 TEST 1 Study Guide
• BCMB 412 Final Exam Study Guide
• basics of library preparation
• Acridine orange
• DNA composition and base pair bonding

Primers

Primers, aka oligonucleotides (oligos), are key in DNA synthesis. They’re small pieces of single-stranded nucleotides, around 5-22 bps in length. Primers must be complementary to DNA strands, which “prime” the strands to allow DNA polymerase to bind and start DNA synthesis

There are DNA and RNA primers, and DNA primers are used in experiments because they’re mot stable. RNA primers are more common in vivo, but DNA primers are used in PCR amplification, DNA sequencing, cloning, etc.

DNA primers vs RNA primers

• Reaction:
  • amplification is temp. dependent, requires less proteins
  • **replication is enzyme dependent catalytic reaction, requires lots of proteins
• Length:
  • 18-24 bps
  • **10-20 bps
• Creation:
  • chemically syntheized (lab made)
  • **primase (type of RNA polymerase) creates them
• Viability:
  • longer shelf-life, more stable
  • **shorter life, more reactive
• Designing PCR Primers

Neurobiology

• gibco-neurobiology-protocol-handbook.pdf

Experimental Methods

• ELISAs
• cell viability assays
• pbmcs are a source of diagnostic and prognostic biomarkers
• Meso Scale Discovery: similar to ELISA, but higher sensitivity, larger detection range, and still efficient at low volume samples. Also has minimized matrix effect, which helps with simultaneous multiplex detection with high precision.

Courses

• GAN 110 Méthodes spectrométriques et biotechnologies, application à la bioanalyse
• BCMB 412 Advanced Molecular Biology and Genomics
• BCMB 415 Foundations of Neurobiology

Websites

• Category: Biotechnology and Research Methods - Biology Insights: great mini-articles and also have no idea who made this. The Biotechnology and Research Methods has condensed articles about specific methods, what they’re used for, and how they work
• Index - Laboratory Notes: I have no idea who writes this, but it’s a gold mine for wetlab biologists and I wish I found this when I first started my internships and knew nothing about bench work.