up:: Career Development & Business MOC tags::
[!info] This MOC focuses on Biological Science MOC and closely associated topics such as Molecular Biology, Neurobiology, Neuroscience, Genetics, and Cellular Biology. Major tags include: science ”/molbio ”/neuro ”/genetics ”/cellbio ”/evol ”/anthro
Cell Bio
- A Beginner’s Guide to Cell Culture
- while antibiotics are convenient, strict aeseptic techniques produce consistently reliable cell culture data
- using panels in bio experiments are efficient and provide a holistic view of a system
- three types of cell lines - finite, continuous or immortalized, and stem cell lines
- the cell cycle
- screening t-cells helps identify potential therapies for diseases
- software FloJo popular for analyzing flow cytometry data
- single-cell sequencing and its use in drug development
- proliferation assays and common issues with pbmcs
- pbmcs are a source of diagnostic and prognostic biomarkers
- neutrophils
- lymphocytes
- macrophages
- layers of the skin
- how to decide if we should use magnetic cell separation of FACS to isolate cells
- gating, forward-scatter cells, side-scatter cells
- cytotoxicity assays and common issues with pbmcs
- cytokine production assays and their common issues
- common standard cell culture media
- class I cytokine receptor
- cell line quality control testing for new cells
- cell isolation, cell separation, and cell sorting can be used for different purposes but are very related
- cancer at a cellular level
- antibodies
- Small Lab Process hacks
- Prolactin signaling
- Merck Organoid and automation event 2025-09-30
- Haematapoietic stem cells (HSCs)
- ELISAs
- Chemometec training
- 3D cell culture and techniques
- 2025-09-25 session 2 - Interferons, Innate Immunity Receptors and cell signalling
- 2025-09-18 session 1 - La réponse immunitaire innée pt1 of 3
- Cellular Communication Systems
- major histocompatibility complex
- BLG 104 Microbiologie, virologie, immunologie
- the potential of bio hacking in self-directed apoptosis
Mol Bio
- Using Snapgene for plasmids and cloning
- Model Organisms Are Not Static - Asimov Press
- A Visual Guide to Genome Editors - by Evan DeTurk
- A Visual Guide to Genome Editors - by Evan DeTurk
- How to Adapt Centrifuge Time for Protocols
- why use a fluorometer instead of a spectrophotometer
- western blot
- ubiquitin and linkage
- transposon
- transcription factor
- topoisomerases prevent supercoiling for accessible replication and transcription
- the glucose effect in bacterial growth
- the VGEF protein and its molecular pathway
- the HER2 protein and pathway
- the 5 properties of genetic codes
- tRNAs and ribosomes
- southwestern blot
- ribozymes (RNase) enable tertiary structure change via intramolecular interactions
- protein dimerization and oligerimization
- probes
- plasmid copy number
- operons, lac operon, trp operon
- nucleotides and chain directionality
- mobility of relaxed, supercoiled and linear forms of a DNA on agarose gel and effects of EtBr
- membrane tyrosine kinase
- ion channels
- how to create dna biochips
- comparing chemical structure of RNA vs DNA
- blotting techniques
- basics of library preparation
- Troubleshooting Checkpoints for Cloning
- Stringent response mechanism
- Restriction Cloning
- RNA pseudoknots and gene regulation
- Polymerase chain reaction (PCR)
- Phosphorelay cascade
- Identifying quality of DNA prep on structureed
- ICM - Assistante Ingénieur
- Designing PCR Primers
- DNA replication is a semiconservative process
- DNA hybridization
- DNA composition and base pair bonding
- DNA Microarrays
- Chargaff’s Rule
- BCMB 412 Test 3 Study Guide
- BCMB 412 Test 2 Study Guide
- BCMB 412 TEST 1 Study Guide
- BCMB 412 Final Exam Study Guide
- BCMB 412 Advanced Molecular Biology and Genomics
- Acridine orange
Primers
Primers, aka oligonucleotides (oligos), are key in DNA synthesis. They’re small pieces of single-stranded nucleotides, around 5-22 bps in length. Primers must be complementary to DNA strands, which “prime” the strands to allow DNA polymerase to bind and start DNA synthesis
There are DNA and RNA primers, and DNA primers are used in experiments because they’re mot stable. RNA primers are more common in vivo, but DNA primers are used in PCR amplification, DNA sequencing, cloning, etc.
DNA primers vs RNA primers
- Reaction:
- amplification is temp. dependent, requires less proteins
- **replication is enzyme dependent catalytic reaction, requires lots of proteins
- Length:
- 18-24 bps
- **10-20 bps
- Creation:
- chemically syntheized (lab made)
- **primase (type of RNA polymerase) creates them
- Viability:
- longer shelf-life, more stable
- **shorter life, more reactive
- Designing PCR Primers
Neurobiology
Experimental Methods
- ELISAs
- cell viability assays
- pbmcs are a source of diagnostic and prognostic biomarkers
- Meso Scale Discovery: similar to ELISA, but higher sensitivity, larger detection range, and still efficient at low volume samples. Also has minimized matrix effect, which helps with simultaneous multiplex detection with high precision.
- luminex assays
- BCA protein assay
- cell viability assays
- OD 600 normalization
Courses
- GAN 110 Méthodes spectrométriques et biotechnologies, application à la bioanalyse
- BCMB 412 Advanced Molecular Biology and Genomics
- BCMB 415 Foundations of Neurobiology
Websites
- Category: Biotechnology and Research Methods - Biology Insights: great mini-articles and also have no idea who made this. The Biotechnology and Research Methods has condensed articles about specific methods, what they’re used for, and how they work
- Index - Laboratory Notes: I have no idea who writes this, but it’s a gold mine for wetlab biologists and I wish I found this when I first started my internships and knew nothing about bench work.